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Proceedings Paper

Observation of photodynamically-induced cell destruction probed by video microscopy, laser-scanning microscopy, and fluorescence spectroscopy
Author(s): Angelika C. Rueck; Wolfgang S. L. Strauss; Michael H. Gschwend; Karsten Koenig; B. Brunner; Herbert Schneckenburger; Heinrich Walt; Rudolf W. Steiner
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Paper Abstract

In order to study light-induced reactions during PDT, the fluorescence response of the photosensitizer meso-tetra(4-sulfonatophenyl)porphyrin (TPPS4) was observed in different cell systems and correlated with the sensitivity to photodynamic induced destructions. RR 1022 epithelial cells from the rat were grown on microscopic slides at a high and low cell density. Using video microscopy in combination with microspectrofluorometry we observed a different fluorescence behavior for high and low cell conditions during light exposure. A fluorescence relocalization from the cytoplasm to the nucleus and an intensity increase-- correlated with the formation of a new molecular species--could be detected only for low cell density. Moreover, cell cultures at a high density showed to be less sensitive to photodynamic destructions. In addition to cell culture-experiments, we observed the light-induced reactions of TPPS4 accumulated in multicellular tumor spheroids. For these measurements laser scanning microscopy was used. Fluorescence relocalization and intensity increase could be detected only for the peripheric parts of the spheroids. The different fluorescence response seems to reflect different metabolic and physiologic states of the cells.

Paper Details

Date Published: 7 July 1993
PDF: 7 pages
Proc. SPIE 1882, Laser-Tissue Interaction IV, (7 July 1993); doi: 10.1117/12.147691
Show Author Affiliations
Angelika C. Rueck, Institut fuer Lasertechnologien in der Medizin, Univ. Ulm (Germany)
Wolfgang S. L. Strauss, Institut fuer Lasertechnologien in der Medizin, Univ. Ulm (Germany)
Michael H. Gschwend, Institut fuer Lasertechnologien in der Medizin, Univ. Ulm (Germany)
Karsten Koenig, Institut fuer Lasertechnologien in der Medizin, Univ. Ulm (Germany)
B. Brunner, Univ. Hospital, Zuerich (Switzerland)
Herbert Schneckenburger, Institut fuer Lasertechnologien in der Medizin, Univ. Ulm (Germany)
Heinrich Walt, Univ. Hospital, Zuerich (Switzerland)
Rudolf W. Steiner, Institut fuer Lasertechnologien in der Medizin, Univ. Ulm (Germany)


Published in SPIE Proceedings Vol. 1882:
Laser-Tissue Interaction IV
Steven L. Jacques; Abraham Katzir, Editor(s)

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