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Proceedings Paper

Photochemical cross-linking of proteins with visible-light-absorbing 1,8-naphthalamides
Author(s): Millard M. Judy; James Lester Matthews; Richard L. Boriak; A. Burlacu; David E. Lewis; Ronald E. Utecht
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Paper Abstract

We have newly synthesized a class of photochemical 1,8-naphthalimide dyes. Photochemical investigation strongly suggests that these dyes function as photoalkylation agents following activation to an intermediate state by visible light (circa 420 nm) excitation. The activated species reacts readily with nucleophilic amino acid residues, e.g., tryptophan, cysteine, and methionine. One dye, 1,14-bis-(N-hexyl-3'-bromo-1,8'-naphthalimid-4'-yl)-1,4,11,14- tetraazatetradecane-5,10-dione, which incorporates two reactive 1,8-naphthalimide groups at each end of an intervening structural bridge has been used to cross-link the protein monomers of F-actin, thus preventing its natural depolymerization at low salt concentrations, and to cross-link Apolipoprotein I of human high-density lipoprotein. These observations suggest continued study of these dyes as agents for protein cross-linking, tissue bonding, and inactivation of infectious agents.

Paper Details

Date Published: 7 July 1993
PDF: 4 pages
Proc. SPIE 1882, Laser-Tissue Interaction IV, (7 July 1993); doi: 10.1117/12.147669
Show Author Affiliations
Millard M. Judy, Baylor Research Institute (United States)
James Lester Matthews, Baylor Research Institute (United States)
Richard L. Boriak, Baylor Research Institute (United States)
A. Burlacu, Baylor Research Institute (United States)
David E. Lewis, South Dakota State Univ. (United States)
Ronald E. Utecht, South Dakota State Univ. (United States)


Published in SPIE Proceedings Vol. 1882:
Laser-Tissue Interaction IV
Steven L. Jacques; Abraham Katzir, Editor(s)

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