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Proceedings Paper

Principles, practice, and instrumentation for optical recording of cellular metabolism
Author(s): Barry R. Masters
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Paper Abstract

This chapter describes the principles and practice of the optical recording of cellular metabolism. Redox imaging of cells, tissues, and organs based on intrinsic fluorescent probes of cellular metabolism. Cellular metabolism may be noninvasively interrogated through the `optical method' based on the fluorescence intensity of intrinsic probe molecules (Change, 1991). The intrinsic fluorescent probes, which report on cellular metabolism, are the reduced pyridine nucleotides, NAD(P)H, and the oxidized flavoproteins.

Paper Details

Date Published: 24 June 1993
PDF: 9 pages
Proc. SPIE 1895, Ultrasensitive Laboratory Diagnostics, (24 June 1993); doi: 10.1117/12.146727
Show Author Affiliations
Barry R. Masters, Uniformed Services Univ. of the Health Sciences (United States)


Published in SPIE Proceedings Vol. 1895:
Ultrasensitive Laboratory Diagnostics
Gerald E. Cohn, Editor(s)

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