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Proceedings Paper

Fluorescence-lifetime-based sensing: applications to clinical chemistry and cellular imaging
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Paper Abstract

Measurements of fluorescence lifetimes, rather than intensity or intensity ratios, offer many advantages in clinical chemistry and imaging. However, measurements of time-resolved fluorescence are normally associated with complex laser light sources and instrumentation. In this lecture, we show how emerging technology is enabling the design and use of simple instrumentation for time-resolved fluorescence. In particular, it is now possible to imagine lifetime-based measurements of blood gases and blood glucose, and lifetime imaging of calcium and other ions in microscopic samples.

Paper Details

Date Published: 24 June 1993
PDF: 16 pages
Proc. SPIE 1895, Ultrasensitive Laboratory Diagnostics, (24 June 1993); doi: 10.1117/12.146718
Show Author Affiliations
Joseph R. Lakowicz, Univ. of Maryland/Baltimore School of Medicine (United States)
Henryk Szmacinski, Univ. of Maryland/Baltimore School of Medicine (United States)
Richard B. Thompson, Univ. of Maryland/Baltimore School of Medicine (United States)


Published in SPIE Proceedings Vol. 1895:
Ultrasensitive Laboratory Diagnostics
Gerald E. Cohn, Editor(s)

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