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Proceedings Paper

Development of resonance ionization spectroscopy for genome mapping and DNA sequencing using stable isotopes as DNA labels
Author(s): K. Bruce Jacobson; Heinrich F. Arlinghaus; Mitchel J. Doktycz; R. A. Sachleben; Gilbert M. Brown; F. W. Larimer
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Paper Abstract

Stable isotopes can be used as alternatives to radioisotopes or fluorescent DNA labels. After the labeled DNA has been banded on electrophoresis gels the stable isotopes may be located by scanning the gel with resonance ionization spectroscopy. Methods for synthesizing appropriate labels and for detecting isotopes of iron and tin directly on polyacrylamide gels are described.

Paper Details

Date Published: 24 June 1993
PDF: 10 pages
Proc. SPIE 1891, Advances in DNA Sequencing Technology, (24 June 1993); doi: 10.1117/12.146716
Show Author Affiliations
K. Bruce Jacobson, Oak Ridge National Lab. (United States)
Heinrich F. Arlinghaus, Atom Sciences, Inc. (United States)
Mitchel J. Doktycz, Oak Ridge National Lab. (United States)
R. A. Sachleben, Oak Ridge National Lab. (United States)
Gilbert M. Brown, Oak Ridge National Lab. (United States)
F. W. Larimer, Oak Ridge National Lab. (United States)

Published in SPIE Proceedings Vol. 1891:
Advances in DNA Sequencing Technology
Richard A. Keller, Editor(s)

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