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Proceedings Paper

Capillary array electrophoresis: an approach to high-speed high-throughput DNA sequencing
Author(s): Xiaohua C. Huang; Mark A. Quesada; Richard A. Mathies
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Paper Abstract

A laser-excited, confocal-fluorescence scanner is used for high-sensitivity, on-column detection of electrophoresis performed using an array of capillaries. The capabilities of this method and apparatus are illustrated by application to high-speed, high-throughput DNA sequencing. Sanger DNA sequencing fragments are separated on an array of capillaries and then distinguished by using a binary coding scheme that employs only two different fluorescently labeled dye primers to identify four sets of fragments. DNA sequencing results are presented using a 25-capillary array. This apparatus has the capability of sequencing DNA at a rate of approximately 25,000 bases/hour.

Paper Details

Date Published: 24 June 1993
PDF: 9 pages
Proc. SPIE 1891, Advances in DNA Sequencing Technology, (24 June 1993); doi: 10.1117/12.146714
Show Author Affiliations
Xiaohua C. Huang, Univ. of California/Berkeley (United States)
Mark A. Quesada, Univ. of California/Berkeley (United States)
Richard A. Mathies, Univ. of California/Berkeley (United States)

Published in SPIE Proceedings Vol. 1891:
Advances in DNA Sequencing Technology
Richard A. Keller, Editor(s)

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