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Proceedings Paper

Accuracy of two-color peak-height-encoded DNA sequencing by capillary gel electrophoresis and laser-induced fluorescence
Author(s): Sue Bay; Heather Starke; John Elliott; Norman J. Dovichi
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Paper Abstract

The two-color peak-height encoded DNA sequencing technique was evaluated with six clones taken from the malaria genome. This technique produced a sequencing accuracy of at least 97.5%. Capillary gel electrophoresis, using a modest voltage of 200 V/cm, showed a three- fold increase in speed and higher efficiency compared to conventional slab gel technique.

Paper Details

Date Published: 24 June 1993
PDF: 4 pages
Proc. SPIE 1891, Advances in DNA Sequencing Technology, (24 June 1993); doi: 10.1117/12.146713
Show Author Affiliations
Sue Bay, Univ. of Alberta (Canada)
Heather Starke, Univ. of Alberta (Canada)
John Elliott, Univ. of Alberta (Canada)
Norman J. Dovichi, Univ. of Alberta (Canada)

Published in SPIE Proceedings Vol. 1891:
Advances in DNA Sequencing Technology
Richard A. Keller, Editor(s)

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