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Proceedings Paper

Matrix- and substrate-assisted laser desorption for fast DNA sequencing
Author(s): C. H. Winston Chen; S. L. Allman; K. Tang; R. B. Jones
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Paper Abstract

During the past few years, tremendous effort has been put to achieving fast sequencing of DNA due to the potential great applications in biological and medical research. Current successful DNA sequencing methods which were developed by Sanger and Maxam-Gilbert have proven very useful for sequencing small DNA segments. However, these methods of sequencing usually involve labeling fragments of DNA for identification following time- consuming gel electrophoresis. The labeling processes usually involve either radioactive tagging or chromophore tagging to various sizes of DNA segments. The segments of DNA are generated either chemically or enzymatically to represent all possible positions of each of the four nucleotides (A, G, C, and T). Both radioactive methods and fluorescent dye labeling methods for DNA sequencing require the use of the time-consuming gel electrophoresis method. It is naturally desirable to consider a time-of-flight mass spectrometer approach to DNA sequencing since the time needed for separation of DNA segments in a mass spectrometer is a few microseconds instead of a few hours in gel electrophoresis.

Paper Details

Date Published: 24 June 1993
PDF: 8 pages
Proc. SPIE 1891, Advances in DNA Sequencing Technology, (24 June 1993); doi: 10.1117/12.146709
Show Author Affiliations
C. H. Winston Chen, Oak Ridge National Lab. (United States)
S. L. Allman, Oak Ridge National Lab. (United States)
K. Tang, Oak Ridge National Lab. (United States)
R. B. Jones, Oak Ridge National Lab. (United States)


Published in SPIE Proceedings Vol. 1891:
Advances in DNA Sequencing Technology
Richard A. Keller, Editor(s)

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