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Proceedings Paper

Sequencing using pulsed field and image reconstruction
Author(s): Jaan Noolandi; Robert Forsyth; Chantal Turmel; An-Chang Shi
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Paper Abstract

The use of pulsed fields in a standard manual sequencing set-up results in the separation of over 2000 bases on a single gel. However visual reading of the sequence from a film is not possible for more than about 800 - 900 bases under the best conditions. The use of image reconstruction and analysis techniques allows the reading of the M13 mp18 sequence up to about 1600 - 1700 bases, and individual bands can be identified above 2000 bases.

Paper Details

Date Published: 24 June 1993
PDF: 6 pages
Proc. SPIE 1891, Advances in DNA Sequencing Technology, (24 June 1993); doi: 10.1117/12.146702
Show Author Affiliations
Jaan Noolandi, Xerox Research Ctr. of Canada (Canada)
Robert Forsyth, Xerox Research Ctr. of Canada (Canada)
Chantal Turmel, Xerox Research Ctr. of Canada (Canada)
An-Chang Shi, Xerox Research Ctr. of Canada (Canada)

Published in SPIE Proceedings Vol. 1891:
Advances in DNA Sequencing Technology
Richard A. Keller, Editor(s)

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