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Proceedings Paper

Scanning force microscopy and STM imaging of B-chitin
Author(s): Manfred H. Jericho; P. J. Mulhern; Wenbo Xu; B. L. Blackford; Lawrence Fritz
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Paper Abstract

A combination of SEM, STM, and SFM microscopy determined structural and elastic properties of (beta) -chitin, a linear polysaccharide that serves as a skeletal material in some marine species. Each microscopy technique produced artifacts that may lead to incorrect conclusions, but consistent results from multiple techniques improve the confidence in the interpretation. Our studies confirmed the existence of 40 nm X 20 - 50 nm substructures in chitin fibers from the diatom Thalassiosira fluviatilis, and found several surface structures not previously reported. We also investigated a technique to enhance subtle features in images by vibrating an SFM sample stage. The SFM directly measured the Young's Modulus of the fibers to be 1 +/- 0.5 X 1011 N/m2.

Paper Details

Date Published: 4 June 1993
PDF: 9 pages
Proc. SPIE 1855, Scanning Probe Microscopies II, (4 June 1993); doi: 10.1117/12.146379
Show Author Affiliations
Manfred H. Jericho, Dalhousie Univ. (Canada)
P. J. Mulhern, Dalhousie Univ. (Canada)
Wenbo Xu, Dalhousie Univ. (Canada)
B. L. Blackford, Dalhousie Univ. (Canada)
Lawrence Fritz, Institute for Marine Biology/NRC (Canada)

Published in SPIE Proceedings Vol. 1855:
Scanning Probe Microscopies II
Clayton C. Williams, Editor(s)

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