Share Email Print
cover

Proceedings Paper

Electron microscopy study of tumor destruction by photodynamic therapy
Author(s): Mladen Korbelik; Jasenka Matisic; Gorazd Krosl
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Sections of SCCVII tumor (squamous cell carcinoma) grown in C3H mice treated with Photofrin based photodynamic therapy (PDT) were examined by electron microscopy. The tumors were collected at different times after light treatment, and divided according to light penetration into superficial (treatment light oriented) and deep areas. The results show evidence of the progressive destruction of tumor cells occurring in both areas, with the presence of apparently intact tumor cells in the deep region 6 hours after PDT. These healthy looking tumor cells were often found adjacent to heavily damaged tumor cells, which is indicative of selectivity in their killing within the same microregion of tumor tissue. Many histiocytes and mast cells that appeared engaged in tumoricidal activity were also detected. It is suggested that in addition to lethality coming from progressive hemorrhagic necrosis, host immune cells infiltrating the tumor are elicited by PDT and contribute to the indirect killing effect.

Paper Details

Date Published: 18 June 1993
PDF: 8 pages
Proc. SPIE 1881, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy II, (18 June 1993); doi: 10.1117/12.146323
Show Author Affiliations
Mladen Korbelik, British Columbia Cancer Research Ctr. (Canada)
Jasenka Matisic, British Columbia Cancer Agency (Canada)
Gorazd Krosl, British Columbia Cancer Research Ctr. (Canada)


Published in SPIE Proceedings Vol. 1881:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy II
Thomas J. Dougherty, Editor(s)

© SPIE. Terms of Use
Back to Top