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Proceedings Paper

Biodistribution of benzoporphyrin derivative in tumor-bearing rats by laser-induced fluorescence spectroscopy
Author(s): Sandor G. Vari; Marigo Stavridi; Thanassis Papaioannou; Theodore G. Papazoglou; Vani R. Pergadia; Michael C. Fishbein; David Wolfson; Warren S. Grundfest
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Paper Abstract

The goal of this study was to detect the presence of benzoporphyrin derivative-monoacid (BPD-MA) in tissues of a tumor bearing animal model. Eighty one Lobund-Wistar rats, inoculated with Pollard rat adenocarcinoma cells, were used. This animal model exhibits unique predictable, unilateral, metastatic spread. The animals were injected intravenously with 0.75 mg/kg of BPD-MA. A Helium-Cadmium (He-Cd) laser (442 nm, 17 mW) was used as an excitation source and coupled to a 400 micrometers core diameter fiber. Following laparotomy, exploration of the abdominal and inguinal area was performed with laser induced fluorescence. Fluorescence spectra of the primary tumor, bilateral lymph nodes, and various organs were recorded. Fluorescence measurements were conducted four hours post injection. The spectra obtained were characterized by a broadband autofluorescence (approximately 540 nm) and a characteristic peak of BPD-MA (approximately 690 nm). Overall, the BPD-MA concentration was higher in lymph nodes than in the skin, kidney, large bowel, muscle or spleen. Skin exhibited the lowest fluorescence intensity ratio, indicative of a lower drug concentration in this tissue. In summary, our results suggest that laser induced fluorescence spectroscopy may provide an alternative way of assessing the biodistribution of BPD-MA or other photosensitizers.

Paper Details

Date Published: 18 June 1993
PDF: 11 pages
Proc. SPIE 1881, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy II, (18 June 1993); doi: 10.1117/12.146311
Show Author Affiliations
Sandor G. Vari, Cedars-Sinai Medical Ctr. (United States)
Marigo Stavridi, Cedars-Sinai Medical Ctr. (Netherlands)
Thanassis Papaioannou, Cedars-Sinai Medical Ctr. (United States)
Theodore G. Papazoglou, Institute of Electronic Structure and Laser (Greece)
Vani R. Pergadia, Cedars-Sinai Medical Ctr. (United States)
Michael C. Fishbein, Cedars-Sinai Medical Ctr. (United States)
David Wolfson, Cedars-Sinai Medical Ctr. (Denmark)
Warren S. Grundfest, Cedars-Sinai Medical Ctr. (United States)

Published in SPIE Proceedings Vol. 1881:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy II
Thomas J. Dougherty, Editor(s)

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