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Proceedings Paper

Liposome-administered tetramethylhematoporphyrin (TMHP) as a photodynamic agent for bladder tumor cells
Author(s): Ella Dumbravia Reich; Ruediger Bachor; Kurt Miller; Karsten Koenig; Richard E. Hautmann
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Paper Abstract

This study was made in order to determine whether liposomes can bind and deliver the photosensitizer to human bladder carcinoma cells and how effective the photodynamic activity of this photosensitizer is. TMHP (synthesized by Prof. Muller v.d. Haegen) was incorporated into small unilamellar vesicles of DPPC, following the procedure described by Jori et al TMHP was used in a dosage of 2.5, 5, 10, and 20 (mu) g/ml on two different cell lines. Cellular uptake of TMHP in liposomes was observed by fluorescence microscopy. Dark toxicity became evident, when doses of 10 and 20 (mu) g/ml TMHP encapsulated in liposomes were compared to control liposomes without photosensitizer. PDT was performed after sensitization of cells for one hour using an argon-pumped dye laser at 630 nm and a power density of 30 mW/cm2. Irradiation with 3,6 and 7,2 Joule/cm2 resulted in a decreasing survival rate. This study demonstrates PDT-efficiency being dependent on the dose of liposome-encapsulated TMHP as well as the fluence rate. There is also a difference in cell survival according to the cell line.

Paper Details

Date Published: 18 June 1993
PDF: 15 pages
Proc. SPIE 1881, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy II, (18 June 1993); doi: 10.1117/12.146302
Show Author Affiliations
Ella Dumbravia Reich, Univ. Ulm (Germany)
Ruediger Bachor, Univ. Ulm (Germany)
Kurt Miller, Univ. Ulm (Germany)
Karsten Koenig, Univ. Ulm (Germany)
Richard E. Hautmann, Univ. Ulm (Germany)


Published in SPIE Proceedings Vol. 1881:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy II
Thomas J. Dougherty, Editor(s)

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