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Proceedings Paper

Effect of repeated insertions of curved sequences in DNA plasmids: a light-scattering study
Author(s): Stefano Beretta; Giuseppe Chirico; Giancarlo Baldini; U. Kapp; G. Badaracco
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Paper Abstract

The effect of the insertion of different amounts (from 0 to 6) of the curved sequence AluI in pUC18m plasmid (2686 base pairs, bp) is studied by dynamic light scattering. This sequence is a highly repeated 113 base pairs long sequence from Artemia Franciscana shrimp. A 30% compaction of the plasmids containing 2 and 6 adjacent AluI sequences compared to pUC8 plasmid (2717 bp) is observed. Furthermore the behavior of the translational diffusion coefficient Dt versus the number of adjacent AluI insertion is not monotonic.

Paper Details

Date Published: 17 June 1993
PDF: 5 pages
Proc. SPIE 1922, Laser Study of Macroscopic Biosystems, (17 June 1993); doi: 10.1117/12.146215
Show Author Affiliations
Stefano Beretta, Univ. degli Studi di Milano (Italy)
Giuseppe Chirico, Univ. degli Studi di Milano (Italy)
Giancarlo Baldini, Univ. degli Studi di Milano (Italy)
U. Kapp, European Molecular Biology Lab. (France)
G. Badaracco, Univ. degli Studi di Milano (Italy)


Published in SPIE Proceedings Vol. 1922:
Laser Study of Macroscopic Biosystems

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