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Proceedings Paper

Laser-mediated variations in merocyanine 540 incorporation into phospholipid membranes
Author(s): Libusa Sikurova; R. Frankova; Jan Psenica; Dusan Chorvat
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Paper Abstract

The influence of Nd/YAG laser irradiation (532 nm) on the MC-540 stained POPC and DMPC liposomes (lipid:dye ratio 42:1 - 20:1) was studied by absorption spectroscopy in the range of 380 - 700 nm. Two absorption bands (533 nm and 567 nm) corresponding to two colored species of the dye -- monomers and dimers -- were observed for MC-540 in phospholipid liposomes. MC-40 monomer-dimer distribution was shown to depend on the liposome composition and lipid:dye ratio. Irradiation of the samples by laser light of fluence rate 3000 W/m2 resulted only in MC-540 monomer-dimer distribution changes. The variation of this effect was observed as the kind of liposomes (alcoholic or ultrasonic) were changed. MC-540 monomer-dimer distribution is one of the factors that affect the dye- mediated photoinactivation. Illumination by intensive laser light (60,000 W/m2) caused color fading of the MC-540 dye.

Paper Details

Date Published: 17 June 1993
PDF: 6 pages
Proc. SPIE 1922, Laser Study of Macroscopic Biosystems, (17 June 1993); doi: 10.1117/12.146193
Show Author Affiliations
Libusa Sikurova, Comenius Univ. (Slovak Republic)
R. Frankova, Comenius Univ. (Slovak Republic)
Jan Psenica, Comenius Univ. (Slovak Republic)
Dusan Chorvat, Comenius Univ. (Slovak Republic)

Published in SPIE Proceedings Vol. 1922:
Laser Study of Macroscopic Biosystems

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