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Proceedings Paper

Manipulation and characterization of cells by laser light
Author(s): Jan Greve; Richard M.P. Doornbos; Tom C. Bakker Schut; Bart G. de Grooth
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Paper Abstract

Both for medical and biotechnological purposes it is very important to be able to analyze and manipulate cellular populations on a single cell basis. Moreover it is often important to be able to sort a subfraction of cells from the total population with defined specific characteristics. Since the retainment of biological viability is of importance in such cases the use of monoclonal antibodies for cell characterization cannot always be applied. The use of light scattering offers an attractive alternative. One of the manipulations which is at the beginning of many biotechnological processes is the fusion between two well characterized and differing cell types. In the present paper we concentrate on the use of flowcytometry for light scattering characterization and fusion of cells.

Paper Details

Date Published: 17 June 1993
PDF: 4 pages
Proc. SPIE 1922, Laser Study of Macroscopic Biosystems, (17 June 1993); doi: 10.1117/12.146190
Show Author Affiliations
Jan Greve, Univ. of Twente (Netherlands)
Richard M.P. Doornbos, Univ. of Twente (Netherlands)
Tom C. Bakker Schut, Univ. of Twente (Netherlands)
Bart G. de Grooth, Univ. of Twente (Netherlands)

Published in SPIE Proceedings Vol. 1922:
Laser Study of Macroscopic Biosystems
Jouko E. Korppi-Tommola, Editor(s)

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