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Proceedings Paper

Diffusion of single molecules through a Gaussian laser beam
Author(s): Rudolf Rigler; Ulo Mets
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Paper Abstract

The diffusion of a single molecule through a sharply focused laser beam has been observed. The diffusion was observed by recording photoelectron bursts of single molecules in transit through the laser beam with Gaussian intensity profile. Up to 20 photoelectrons were detected for Rhodamin 6G in a transit time of 40 .ts which corresponds to the diffusion time through a volume element of 2x10'61. From the intensity correlation the probability for the occurence of one molecule in the volume element of observation is determined. The existence of one molecule events is also verified by the measured and calculated photoelectron number distribution.

Paper Details

Date Published: 17 June 1993
PDF: 10 pages
Proc. SPIE 1921, Laser Spectroscopy of Biomolecules, (17 June 1993); doi: 10.1117/12.146154
Show Author Affiliations
Rudolf Rigler, Karolinska Institut (Sweden)
Ulo Mets, Karolinska Institut (Sweden)

Published in SPIE Proceedings Vol. 1921:
Laser Spectroscopy of Biomolecules
Jouko E. Korppi-Tommola, Editor(s)

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