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Proceedings Paper

Near-infrared FT-Raman spectroscopy of living photosynthetic bacteria
Author(s): Yukihiro Ozaki; H. Sato; K. Okada; Y. Koyama; K. Uehara
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Paper Abstract

Near-infrared (NIR) excited FT-Raman spectra were measured in vivo for whole living photosynthetic bacteria. Rhodobacter (Rb.) sphaeroides G1C and R26 and Chlorobium (C.) limicola f. thiosulfatophilum. The obtained spectra, which were preresonanced with the Qy bands of bacteriochlorophyll-a (BChl-a) or bacteriochlorophyll-c (BChl-c), did not contain contributions from major components of the bacteria such as proteins and lipids, and therefore, provided selectively structural information about BChl-a in the light-harvesting (LH) complexes (Rb. sphaeroides) or BChl-c in the chlorosome (C. limicola f. thiosulfatophilum) in a totally nondestructive manner.

Paper Details

Date Published: 17 June 1993
PDF: 8 pages
Proc. SPIE 1921, Laser Spectroscopy of Biomolecules, (17 June 1993); doi: 10.1117/12.146135
Show Author Affiliations
Yukihiro Ozaki, Kwansei Gakuin Univ. (Japan)
H. Sato, Kwansei Gakuin Univ. (Japan)
K. Okada, Kwansei Gakuin Univ (Japan)
Y. Koyama, Kwansei Gakuin Univ. (Japan)
K. Uehara, Univ. of Osaka Prefecture (Japan)

Published in SPIE Proceedings Vol. 1921:
Laser Spectroscopy of Biomolecules
Jouko E. Korppi-Tommola, Editor(s)

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