Share Email Print
cover

Proceedings Paper

Study of pyruvate decarboxylase and thiamine kinase from brewer's yeast by SERS
Author(s): Sergei A. Maskevich; Ivan P. Chernikevich; Gennedy A. Gachko; Leonid N. Kivach; Nataliya D. Strekal
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

The Surface Enhanced Raman Scattering (SERS) spectra of holopyruvate decarboxylase (PDC) and thiamine kinase (ThK) adsorbed on silver electrode were obtained. In contrast to the Raman, the SERS spectrum of PDC contained no modes of tryptophan residues, it indicates a removal of this moiety from the surface. In the SERS spectrum of ThK the bands belonging to ligands bound to the protein were observed. A correlation between the SERS signal intensity and the enzymatic activity of the ThK separate fraction and found. The influence of amino acids on SERS spectra of thiamine (Th) was studied to determine the possible composition on microsurrounding of coenzyme.

Paper Details

Date Published: 17 June 1993
PDF: 7 pages
Proc. SPIE 1921, Laser Spectroscopy of Biomolecules, (17 June 1993); doi: 10.1117/12.146134
Show Author Affiliations
Sergei A. Maskevich, Univ. of Grodno (Belarus)
Ivan P. Chernikevich, Institute of Biochemistry (Belarus)
Gennedy A. Gachko, Univ. of Grodno (Belarus)
Leonid N. Kivach, Univ. of Grodno (Belarus)
Nataliya D. Strekal, Univ. of Grodno (Belarus)


Published in SPIE Proceedings Vol. 1921:
Laser Spectroscopy of Biomolecules

© SPIE. Terms of Use
Back to Top