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Proceedings Paper

Phthalocyanine interaction with cells: kinetics of the photodynamic damage and intracellular localization
Author(s): Elena B. Chernyaeva; Jan Greve; Bart G. de Grooth; A. G.J.M. Van Leeuwen; Marina Yu. Poroshina; L. V. Zhorina
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Paper Abstract

Phthalocyanine interaction with cultured cells was studied by means of flow-cytometry, laser scanning confocal microscopy, fluorescent and transmission pH-microphotometry. Irradiation of Pc-loaded cells caused the following order of events: increase of Pc intracellular fluorescenceyieldsmitochondria damageyieldschanges of cell surface and/or volumeyields-plasma membrane potential disintegrationyields-severe damage of the cell plasma membrane and nuclear envelope. For all these processes it takes a few hours to reach a stationary value. Pc inside the cells is found in granules surrounding the Golgi apparatus and in the peripheral cytoplasmic region, last ones partially coinciding with the acidic cellular compartments. A temporary decrease of cytoplasmic and lysosomal pH is caused by Pc uptake and irradiation. Artificial acidation of the cytoplasm of Pc-loaded cells resulted in the enhancement of the efficiency of the photodynamic inactivation.

Paper Details

Date Published: 17 June 1993
PDF: 10 pages
Proc. SPIE 1921, Laser Spectroscopy of Biomolecules, (17 June 1993); doi: 10.1117/12.146130
Show Author Affiliations
Elena B. Chernyaeva, Moscow State Univ. (Russia)
Jan Greve, Univ. of Twente (Netherlands)
Bart G. de Grooth, Univ. of Twente (Netherlands)
A. G.J.M. Van Leeuwen, Univ. of Twente (Netherlands)
Marina Yu. Poroshina, Moscow State Univ. (Russia)
L. V. Zhorina, Moscow State Univ. (Russia)

Published in SPIE Proceedings Vol. 1921:
Laser Spectroscopy of Biomolecules
Jouko E. Korppi-Tommola, Editor(s)

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