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Proceedings Paper

Time-resolved transient absorption and photoresponse studies of oriented bacteriorhodopsin in Langmuir-Blodgett films
Author(s): Helge Lemmetyinen; Marjo Ikonen; Alexey Sharonov; Nikolai V. Tkachenko
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Paper Abstract

In this work we have studied photon initiated processes of bacteriorhodopsin (BR) in Langmuir-Blodget (LB) monolayers and multilayers. As far as we know the present work is the first, where the photoresponse function and kinetics of BR is detected from a monolayer as well as the first report, where oriented monolayers have been used as a proton pump. The transient absorption spectra and the decay functions of multilayers were detected for films in water, in water vapor saturated air, and at room humidity. This had an influence on transient lifetimes. The method we have used to orient the bacteriorhodopsin molecules is based on the LB-technique and the fact that the protein orients during the coating process when a phospholipid binder is used. The photoelectric signals of the monolayers deposited by the different directions had opposite polarities. The amplitude due to five layers was five times as high as that due to a monolayer.

Paper Details

Date Published: 17 June 1993
PDF: 12 pages
Proc. SPIE 1921, Laser Spectroscopy of Biomolecules, (17 June 1993); doi: 10.1117/12.146121
Show Author Affiliations
Helge Lemmetyinen, Univ. of Helsinki (Finland)
Marjo Ikonen, Univ. of Helsinki (Finland)
Alexey Sharonov, Univ. of Helsinki (Finland)
Nikolai V. Tkachenko, Univ. of Helsinki (Finland)

Published in SPIE Proceedings Vol. 1921:
Laser Spectroscopy of Biomolecules
Jouko E. Korppi-Tommola, Editor(s)

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