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Proceedings Paper

Monitoring protein dynamics with time-resolved CD and MCD spectroscopy
Author(s): Robert A. Goldbeck; David S. Kliger
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Paper Abstract

Ellipsometric techniques for time-resolved measurements of visible and near-UV CD and MCD have been introduced in the last few years and used to follow the nanosecond dynamics of protein systems containing suitable chromophores. Recent technical advances are described that permit the extension of fast time-resolved CD measurements into the far-UV (190 - 290 nm) spectral region. This development opens the possibility of using CD spectroscopy to monitor secondary structure changes in real time, at physiological temperatures, during processes such as protein folding. Fast CD techniques also permit the measurement of CD and MCD of photoselected protein chromophores before rotational reorientation restores isotropy. Theoretical expression for the CD and MCD of photoselectively oriented absorbers suggest that additional molecular information might be revealed by such measurements.

Paper Details

Date Published: 1 May 1993
PDF: 8 pages
Proc. SPIE 1890, Biomolecular Spectroscopy III, (1 May 1993); doi: 10.1117/12.145257
Show Author Affiliations
Robert A. Goldbeck, Univ. of California/Santa Cruz (United States)
David S. Kliger, Univ. of California/Santa Cruz (United States)


Published in SPIE Proceedings Vol. 1890:
Biomolecular Spectroscopy III
Laurence A. Nafie; Henry H. Mantsch, Editor(s)

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