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Proceedings Paper

Competitive and sandwich TIRF immunosensors: a model for performance optimization
Author(s): Antonio Schirone; Michele S. Celebre; Claudio Domenici; Danilo De Rossi; Arti Ahluwalia; Lucia Mosiello
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Paper Abstract

The effects of some fundamental parameters on the performance of optical immunosensors based on fluorescence induced by an evanescent wave (total internal reflection fluorescence, or TIRF) are evaluated. Both competitive and sandwich systems are discussed and compared. The performance of such devices are described using the range within which the error is less than a fixed value. Methods of controlling the effects of various parameters are considered.

Paper Details

Date Published: 18 May 1993
PDF: 6 pages
Proc. SPIE 1885, Advances in Fluorescence Sensing Technology, (18 May 1993); doi: 10.1117/12.144737
Show Author Affiliations
Antonio Schirone, ENEA-CRAM (Italy)
Michele S. Celebre, Univ. of Pisa (Italy)
Claudio Domenici, CNR (Italy)
Danilo De Rossi, Univ. of Pisa (Italy)
Arti Ahluwalia, Univ. of Pisa (Italy)
Lucia Mosiello, ENEA-CRE (Italy)

Published in SPIE Proceedings Vol. 1885:
Advances in Fluorescence Sensing Technology
Joseph R. Lakowicz; Richard B. Thompson, Editor(s)

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