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Proceedings Paper

Detection of single molecules in microspheres
Author(s): Michael D. Barnes; William B. Whitten; J. Michael Ramsey; Kim C. Ng; Stephen Arnold
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Paper Abstract

We have investigated the use of micron-sized liquid droplets as sample medium to detect single fluorescent molecules in solution. The use of microdroplets (5 - 15 micrometers diameter) offers several powerful advantages over single-molecule detection schemes involving measurements on bulk liquids where the probe volume is defined by the laser beam. In addition, cavity-quantum electrodynamical (QED) effects have been observed which influence both spontaneous emission rates and fluorescence yields of dye molecules in these microspheres.

Paper Details

Date Published: 18 May 1993
PDF: 8 pages
Proc. SPIE 1885, Advances in Fluorescence Sensing Technology, (18 May 1993); doi: 10.1117/12.144732
Show Author Affiliations
Michael D. Barnes, Oak Ridge National Lab. (United States)
William B. Whitten, Oak Ridge National Lab. (United States)
J. Michael Ramsey, Oak Ridge National Lab. (United States)
Kim C. Ng, California State Univ./Fresno (United States)
Stephen Arnold, Polytechnic Univ. (United States)

Published in SPIE Proceedings Vol. 1885:
Advances in Fluorescence Sensing Technology
Joseph R. Lakowicz; Richard B. Thompson, Editor(s)

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