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Proceedings Paper

Ultratrace analysis of biological substances by capillary electrophoresis/semiconductor laser fluorometry
Author(s): Tetsuhiro Fuchigami; Totaro Imasaka; Masanobu Shiga
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Paper Abstract

In order to simplify a laser fluorometric detector for capillary electrophoresis a semiconductor laser emitting at 660 nm is used as an exciting source. A labeling reagent fluorescent in the deep-red region is newly synthesized and used for determination of amino acids. The separation resolution is several hundred thousand and the detection limit is subattomole levels. Protein is also labeled by the dye developed and is measured at subfemtomole levels. Contrarily, nonfluorescent samples are detected by indirect fluorometry. Amino acids, flavin adenine dinucleotide (FAD), and deoxyadenosine monophosphate (dAMP) are determined, the detection limit being subpicomole levels.

Paper Details

Date Published: 18 May 1993
PDF: 4 pages
Proc. SPIE 1885, Advances in Fluorescence Sensing Technology, (18 May 1993); doi: 10.1117/12.144729
Show Author Affiliations
Tetsuhiro Fuchigami, Kyushu Univ. (Japan)
Totaro Imasaka, Kyushu Univ. (Japan)
Masanobu Shiga, Dojindo Lab. (Japan)

Published in SPIE Proceedings Vol. 1885:
Advances in Fluorescence Sensing Technology
Joseph R. Lakowicz; Richard B. Thompson, Editor(s)

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