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Proceedings Paper

Universal phosphorescence immunoassay
Author(s): Ekaterina Yu. Mantrova; Marina V. Demcheva; Alexander P. Savitsky; Gely V. Ponomarev
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Paper Abstract

Pd-coproporphyrin I (Pd-CP) has optimum phosphorescence characteristics for application in immunoassay. The aim of this study is to work out a universal phosphorescence immunoassay method (UniPhIA) using monoclonal antibodies to Pd-CP and conjugates of various proteins with Pd-CP for detection of insulin. Pd-CP and monoclonal antibodies obtained allow a convenient method for determination of various antigens to be developed, which combines the universal character of the amplified enzyme-linked immunosorbent assay (a-ELISA) with a high sensitivity and the simplicity of the time-resolved phosphorescence immunoassay.

Paper Details

Date Published: 18 May 1993
PDF: 12 pages
Proc. SPIE 1885, Advances in Fluorescence Sensing Technology, (18 May 1993); doi: 10.1117/12.144715
Show Author Affiliations
Ekaterina Yu. Mantrova, Bakh Institute of Biochemistry (Russia)
Marina V. Demcheva, Bakh Institute of Biochemistry (Russia)
Alexander P. Savitsky, Bakh Institute of Biochemistry (Russia)
Gely V. Ponomarev, Bakh Institute of Biochemistry (Russia)

Published in SPIE Proceedings Vol. 1885:
Advances in Fluorescence Sensing Technology
Joseph R. Lakowicz; Richard B. Thompson, Editor(s)

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