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Proceedings Paper

Development and characteristics of a new picosecond fluorescence lifetime system
Author(s): Motoyuki Watanabe; Musubu Koishi; Paul W. Roehrenbeck
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Paper Abstract

A new time-resolved fluorescence measurement system has been developed. A compact streakscope is used as a detector, and the combination of a spectrograph and a streakscope enables the instrument to measure the fluorescence lifetime and spectrum simultaneously using the single photon counting method with a time resolution of 15 ps (5 ps with fitting software). The dynamic range is achieved up to 105. The instrument response function (IRF) of a streakscope is nearly Gaussian, and it has no tail and no `after pulses' which are observed at the IRF of a photomultiplier used in the time correlated single photon counting system (TCPC). So, a short lifetime of several ten picoseconds can be measured with accuracy. In addition, two dimensional (time and wavelength) single photon counting can be performed without scanning the wavelength, so time resolved spectrum can be measured in a short time with a high S/N ratio.

Paper Details

Date Published: 18 May 1993
PDF: 10 pages
Proc. SPIE 1885, Advances in Fluorescence Sensing Technology, (18 May 1993); doi: 10.1117/12.144707
Show Author Affiliations
Motoyuki Watanabe, Hamamatsu Photonics K.K. System (Japan)
Musubu Koishi, Hamamatsu Photonics K.K. System (Japan)
Paul W. Roehrenbeck, Hamamatsu Photonic Systems Corp. (United States)

Published in SPIE Proceedings Vol. 1885:
Advances in Fluorescence Sensing Technology
Joseph R. Lakowicz; Richard B. Thompson, Editor(s)

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