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Proceedings Paper

Ultraviolet-laser-induced fluorescence of aerosolized bacterial spores
Author(s): Steven D. Christesen; Anna Wong; Michael Scott DeSha; Clifton N. Merrow; Mark W. Wilson; John Charles Butler
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Paper Abstract

Until quite recently the ability to detect and discriminate aerosolized micro-organisms at long range using the laser induced fluorescence (LIF) technique has met with limited success. The lasers which met our logistic requirements had insufficient energies to propagate through the troposphere and excite a target organism. The detectors, though sensitive enough, did not allow us to see a spectral distribution of the fluorescence return. Advances in laser and detector technology has now brought us higher energy, solid state lasers, and very sensitive array detectors. Using this new technology we built and tested an ultraviolet LIDAR against various interferents and a micro-organic contaminant. In this paper we describe the system and method used to detect and discriminate an aerosolized micro-organism at ranges up to 3 kilometers, and the results of this effort.

Paper Details

Date Published: 18 May 1993
PDF: 8 pages
Proc. SPIE 1885, Advances in Fluorescence Sensing Technology, (18 May 1993); doi: 10.1117/12.144702
Show Author Affiliations
Steven D. Christesen, U.S. Army Chemical Research, Development, and Engineering Ctr. (United States)
Anna Wong, U.S. Army Chemical Research, Development, and Engineering Ctr. (United States)
Michael Scott DeSha, U.S. Army Chemical Research, Development, and Engineering Ctr. (United States)
Clifton N. Merrow, Science and Technology Corp. (United States)
Mark W. Wilson, Science and Technology Corp. (United States)
John Charles Butler, Univ. of Delaware (United States)

Published in SPIE Proceedings Vol. 1885:
Advances in Fluorescence Sensing Technology
Joseph R. Lakowicz; Richard B. Thompson, Editor(s)

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