Share Email Print
cover

Proceedings Paper

Submicron optical sources for single macromolecule detection
Author(s): Robert J. G. Carr; Sumaia Al-Shukri; Elaine A. Perkins; Thierry Rodard; Tony Atkinson
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Nanobit holographic techniques, such as high-energy electron beam lithography, allow apertures of < 50 nm diam. to be fabricated in the metal coating of SPR sensor devices. When the SPR slab waveguide is edge illuminated with a laser, the apertures act as intense scattering centers of the underlying radiation, the scattered light from which is readily detectable by conventional optical microscopy. Given the dimensions of these sub-wavelength optical sources are within an order of magnitude of those of individual biological macromolecules, it should ultimately prove possible to detect interactions between substrate/analyte and individual (labelled) biological macromolecules immobilized in the apertures. The possibility of analyzing the dynamic behavior of single biomacromolecules operating in their natural environment is discussed.

Paper Details

Date Published: 30 April 1993
PDF: 5 pages
Proc. SPIE 1796, Chemical, Biochemical, and Environmental Fiber Sensors IV, (30 April 1993); doi: 10.1117/12.143509
Show Author Affiliations
Robert J. G. Carr, PHLS Ctr. for Applied Microbiology and Research (United Kingdom)
Sumaia Al-Shukri, PHLS Ctr. for Applied Microbiology and Research (United Kingdom)
Elaine A. Perkins, PHLS Ctr. for Applied Microbiology and Research (United Kingdom)
Thierry Rodard, PHLS Ctr. for Applied Microbiology and Research (United Kingdom)
Tony Atkinson, PHLS Ctr. for Applied Microbiology and Research (United Kingdom)


Published in SPIE Proceedings Vol. 1796:
Chemical, Biochemical, and Environmental Fiber Sensors IV
Robert A. Lieberman, Editor(s)

© SPIE. Terms of Use
Back to Top