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Proceedings Paper

Laser photoacoustic trace detection of C2H4 revealing adverse environmental effects of atmospheric pollution on plant material
Author(s): Frans J. M. Harren; Luciana Petruzzelli
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Paper Abstract

The photoacoustic detection method for trace gases in the atmosphere is well developed towards very low limits of detection, in the last years. Due to the combination of a sensitive photoacoustic cell placed intracavity in an infrared CO2 laser we were able to detect C2H4 at ultralow (< 1:1011) concentrations within 10 seconds, C2H4 in a plant hormone which seems to play an important role throughout all the life stages of a plant, including seed germination. In addition, various types of stress have been reported to promote ethylene production from different plant tissues. As part of our ongoing research on the role of ethylene in seed germination, we have compared our laser photoacoustic set-up to a gaschromatograph for measuring C2H4 produced by germinating Pisum sativum L. seeds within the first days of imbibition. C2H4 evolution by intact seeds shows a maximum at about 25 hours of germination. Thereafter, the rate of ethylene measured by gaschromatograph continues to decrease while that measured by the laser-driven photoacoustic system shows further increases. Most of the ethylene produced by seeds is found in isolated embryonic axes. The fumigation with ozone affects the growth of seedlings and their ethylene evolution.

Paper Details

Date Published: 9 March 1993
PDF: 10 pages
Proc. SPIE 1716, International Conference on Monitoring of Toxic Chemicals and Biomarkers, (9 March 1993); doi: 10.1117/12.140249
Show Author Affiliations
Frans J. M. Harren, Univ. of Nijmegen (Netherlands)
Luciana Petruzzelli, Istituto Germoplasma/CNR (Italy)

Published in SPIE Proceedings Vol. 1716:
International Conference on Monitoring of Toxic Chemicals and Biomarkers
Tuan Vo-Dinh; Karl Cammann, Editor(s)

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