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Proceedings Paper

Development of 50 x 50 fiber-array photodiode camera
Author(s): Mutsuji Takahashi; H. Tsuchiya; Shigeru Hosoi; Tsuyoshi Hayakawa
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Paper Abstract

Optical imaging of neural network activity using voltage sensitive dyes is expected to be easier than the current electrode method to get multiple point information simultaneously. A high- speed camera is needed to capture a change of a fluorescence image caused by a potential change accompanying neuronal activity. The electrical excitation of a neuron lasts for a few milliseconds and the concomitant fluorescence change is reported to be a few percent or less of its total intensity. A 50 X 50 photodiode camera is specifically designed for this purpose. This camera can take pictures with a 12 bit accuracy every 256 microsecond(s) for about 1 sec(4096 pictures). The epi-illuminated fluorescence image through an objective lens is focused on the end-plate of a 50 X 50 plastic optical fiber bundle. Individual photodiodes and ac-coupled amplifiers are connected to each optical fiber. The time-dependent portion of each signal is transferred to one of the ten 12-bit AD converters through a three-stage multiplexer, and digitally stored in a 20 MBytes RAM. We demonstrated the ability of the camera by capturing membrane potential images of sea urchin eggs induced by an external electric field.

Paper Details

Date Published: 19 January 1993
PDF: 4 pages
Proc. SPIE 1757, Ultrahigh- and High-Speed Photography, Videography, and Photonics, (19 January 1993); doi: 10.1117/12.139123
Show Author Affiliations
Mutsuji Takahashi, Hamamatsu Photonics Central Research Lab. (Japan)
H. Tsuchiya, Hamamatsu Photonics Central Research Lab. (Japan)
Shigeru Hosoi, Hamamatsu Photonics Tsukuba Research Lab. (Japan)
Tsuyoshi Hayakawa, Hamamatsu Photonics Central Research Lab. (Japan)


Published in SPIE Proceedings Vol. 1757:
Ultrahigh- and High-Speed Photography, Videography, and Photonics
Donald R. Snyder, Editor(s)

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