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Proceedings Paper

New data inversion formula in confocal scanning microscopy
Author(s): Christine De Mol; Michel Defrise
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Paper Abstract

Whereas the resolving power of an ordinary optical microscope is determined by the classical Rayleigh distance, significant super-resolution, i.e. resolution improvement beyond that Rayleigh limit, has been achieved by confocal scanning light microscopy. Furthermore is has been shown that the resolution of a confocal scanning microscope can still be significantly enhanced by measuring, for each scanning position, the full diffraction image by means of an array of detectors and by inverting these data to recover the value of the object at the focus. We discuss the associated inverse problem and show how to generalize the data inversion procedure by allowing, for reconstructing the object at a given point, to make use also of the diffraction images recorded at other scanning positions. This leads us to a whole family of generalized inversion formulae, which contains as special cases some previously known formulae. We also show how these exact inversion formulae can be implemented in practice.

Paper Details

Date Published: 29 December 1992
PDF: 11 pages
Proc. SPIE 1767, Inverse Problems in Scattering and Imaging, (29 December 1992); doi: 10.1117/12.139004
Show Author Affiliations
Christine De Mol, Univ. Libre de Bruxelles (Belgium)
Michel Defrise, Vrije Univ. Brussels (Belgium)

Published in SPIE Proceedings Vol. 1767:
Inverse Problems in Scattering and Imaging
Michael A. Fiddy, Editor(s)

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