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Proceedings Paper

High-resolution x-ray stereomicroscopy: true three-dimensional imaging of biological samples
Author(s): Billy W. Loo; Shawn P. Williams; W. Lin; Willaim H. Love; Stanley Meizel; Stephen S. Rothman
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Paper Abstract

X-ray microscopy has the potential to become a powerful tool for the study of biological samples, allowing the imaging of intact cells and subcellular organelles in an aqueous environment at resolutions previously achievable only by electron microscopy. The ability to examine a relatively thick sample raises the issue of superposition of objects from multiple planes within the sample, making difficult the interpretation of conventional, orthogonally projected images. This paper describes our early attempts at developing three-dimensional methods for x-ray microimaging: the first to use x-ray optics, and to our knowledge, the first demonstrating sub-visible resolutions and natural contrast. These studies were performed using the scanning transmission x-ray microscope (STXM) at the National Synchrotron Light Source, Brookhaven National Laboratory.

Paper Details

Date Published: 13 January 1993
PDF: 9 pages
Proc. SPIE 1741, Soft X-Ray Microscopy, (13 January 1993); doi: 10.1117/12.138755
Show Author Affiliations
Billy W. Loo, Lawrence Berkeley Lab., Univ. of California/Davis, and Univ. of Ca (United States)
Shawn P. Williams, SUNY/Stony Brook (United States)
W. Lin, Lawrence Berkeley Lab. (United States)
Willaim H. Love, Lawrence Berkeley Lab. (United States)
Stanley Meizel, Univ. of California/Davis (United States)
Stephen S. Rothman, Lawrence Berkeley Lab., Univ. of California/Berkeley, and Univ. of (United States)

Published in SPIE Proceedings Vol. 1741:
Soft X-Ray Microscopy
Chris J. Jacobsen; James E. Trebes, Editor(s)

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