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Proceedings Paper

Measuring changes in the mass of single subcellular organelles using x-ray microscopy
Author(s): Kaarin K. Goncz; Mario M. Moronne; W. Lin; Stephen S. Rothman
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Paper Abstract

Using quantitative scanning transmission x-ray microscopy, zymogen granules isolated from pancreatic acinar cells were observed suspended in aqueous medium at 50 nm resolution. From 3.64 nm x-ray absorption data, the protein content and rate of protein efflux from individual granules were determined. This was accomplished with a specially designed silicon nitride based wet-cell that allowed continuous perfusion and monitoring of individual granules in a variety of different aqueous environments. Granules suspended in 300 mM sucrose, 5 mM phosphate buffer (pH 6.0) were observed to continuously decrease in size and protein content over a period of several hours. Sudden lysis of the granules was not observed. From the flux data, the apparent protein permeability coefficients for individual granules were determined to range from 1 - 10 X 10-10 cm/sec with an average of 4.78 +/- 3.0 X 10-10 cm/sec. We believe this is the first quantitative population profile determined for a subcellular organelle developed from measurements of individual members of the population.

Paper Details

Date Published: 13 January 1993
PDF: 9 pages
Proc. SPIE 1741, Soft X-Ray Microscopy, (13 January 1993); doi: 10.1117/12.138750
Show Author Affiliations
Kaarin K. Goncz, Univ. of California/Berkeley and Lawrence Berkeley Lab. (United States)
Mario M. Moronne, Lawrence Berkeley Lab. (United States)
W. Lin, Lawrence Berkeley Lab. (United States)
Stephen S. Rothman, Lawrence Berkeley Lab. and Univ. of California/San Francisco (United States)


Published in SPIE Proceedings Vol. 1741:
Soft X-Ray Microscopy
Chris J. Jacobsen; James E. Trebes, Editor(s)

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