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Proceedings Paper

Determination by x-ray microscopy of the phases of the x-ray diffraction by macromolecular structures
Author(s): Ronald E. Burge; Christopher J. Buckley; Guy F. Foster; Pauline Bennett
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Paper Abstract

The use of soft x-ray imaging is considered for the determination of the repeating macromolecular structure of biological fibers (e.g., collagen and muscle), within the available image resolution and subject to the effects of radiation damage. A comparison is made between the structure in sarcomere (2 (mu) to 3 (mu) long repeating unit) of striated muscle as seen directly by x-ray microscopy and as derived from published interpretations of x-ray diffraction data from whole muscle. The comparison shows that the loss by radiation damage of the ability of a muscle myofibril to contract is related to the loss of fine structure. Ways to minimize the effects of beam damage are discussed, including the use of images taken in phase, rather than amplitude contrast, and with photon energies above the `water window.'

Paper Details

Date Published: 13 January 1993
PDF: 10 pages
Proc. SPIE 1741, Soft X-Ray Microscopy, (13 January 1993); doi: 10.1117/12.138732
Show Author Affiliations
Ronald E. Burge, King's College London (United Kingdom)
Christopher J. Buckley, King's College London (United Kingdom)
Guy F. Foster, King's College London (United Kingdom)
Pauline Bennett, King's College London (United Kingdom)

Published in SPIE Proceedings Vol. 1741:
Soft X-Ray Microscopy
Chris J. Jacobsen; James E. Trebes, Editor(s)

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