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Proceedings Paper

Time-resolved fluorescence analysis
Author(s): J. B. Alexander Ross; William R. Laws
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Paper Abstract

The choice of fluorescence as an analytical tool is often based on its intrinsic sensitivity. Compounds can be identified on the basis of their steady-state excitation and emission spectra. Resolution and information can be enhanced by the parameters obtained from time-resolved fluorescence. This includes both the intensity decay (lifetimes) and anisotropy decay parameters. For example, often substances have overlapping steady-state excitation and emission spectra. Thus, they can be difficult to resolve. However, these same compounds will often have different fluorescence lifetimes. This allows resolution by excited-state decay kinetics. By testing various associations of the intensity decay parameters with the excitation and emission parameters, as well as with other experimental variables such as pH, temperature, pressure, viscosity, or interaction with other solutes (such as collisional quenchers), one can obtain substantial information about the physical and chemical nature of the sample. In this way, one may 'finger print' complex mixtures, assess compound purity, and characterize a fluorescent compound.

Paper Details

Date Published: 14 August 1992
PDF: 8 pages
Proc. SPIE 1681, Optically Based Methods for Process Analysis, (14 August 1992); doi: 10.1117/12.137726
Show Author Affiliations
J. B. Alexander Ross, Mount Sinai School of Medicine (United States)
William R. Laws, Mount Sinai School of Medicine (United States)


Published in SPIE Proceedings Vol. 1681:
Optically Based Methods for Process Analysis
David S. Bomse; Harry Brittain; Stuart Farquharson; Jeremy M. Lerner; Alan J. Rein; Cary Sohl; Terry R. Todd; Lois Weyer, Editor(s)

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