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Proceedings Paper

Pathological considerations of laser-tissue interactions: light microscopic assessment of thermal damage of skin
Author(s): Thomas J. Flotte; Margaret Goetschkes
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Paper Abstract

A variety of fixatives and stains were examined for the ability to differentially stain the extracellular matrix components of thermal damage to the skin in an attempt to provide methods for examining the extent of thermal effects. This information is important in comparing different lasers and laser parameters. Four zones of thermal damage were identified including char and three zones of less extensive damage. The lower bounds of the damage with steady state conditions for these zones were 64 - 66 degree(s)C, 80 - 85 degree(s)C, and > 100 degree(s)C. The best choices based on this study include the following: fixative: Bouin's, overall stain: H & E, inner zone stain: Pinkus' acid orcein giemsa, middle zone stain: Movat's pentachrome, and outer zone stain: the modified elastic stain presented in the appendix of this paper.

Paper Details

Date Published: 1 June 1992
PDF: 6 pages
Proc. SPIE 1643, Laser Surgery: Advanced Characterization, Therapeutics, and Systems III, (1 June 1992); doi: 10.1117/12.137355
Show Author Affiliations
Thomas J. Flotte, Massachusetts General Hospital and Wellman Labs. of Photomedicine (United States)
Margaret Goetschkes, Massachusetts General Hospital and Wellman Labs. of Photomedicine (United States)


Published in SPIE Proceedings Vol. 1643:
Laser Surgery: Advanced Characterization, Therapeutics, and Systems III
R. Rox Anderson, Editor(s)

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