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Proceedings Paper

Three-dimensional structure of neurons from peripheral autonomic ganglia using confocal microscope images
Author(s): Steven M. Miller; Philip Schmalz; Leonid Ermilov; James Tarara; Ruchun Ma; James Brewer; Joseph H. Szurszewski
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Paper Abstract

An understanding of neuronal physiology at the single unit level requires knowledge of the functional properties of the neurons as well as a detailed description of its morphology. A full morphological description should include a three-dimensional (3-D) analysis. In this paper, we describe for the first time, 3-D morphology of individual neurons of the peripheral autonomic nervous system intact within whole-mount preparations. Neurons were filled with the fluorescent dye Lucifer Yellow and imaged by laser scanning confocal microscopy. Three- dimensional reconstructions were obtained using volume rendering methods on a set of serial optical sections obtained for each neuron. The 3-D images of the neurons we studied showed a complexity of shape and detail that is not readily apparent from images viewed by traditional microscopy.

Paper Details

Date Published: 22 September 1992
PDF: 11 pages
Proc. SPIE 1808, Visualization in Biomedical Computing '92, (22 September 1992); doi: 10.1117/12.131116
Show Author Affiliations
Steven M. Miller, Mayo Clinic (United States)
Philip Schmalz, Mayo Clinic (United States)
Leonid Ermilov, Mayo Clinic (United States)
James Tarara, Mayo Clinic (United States)
Ruchun Ma, Mayo Clinic (United States)
James Brewer, Mayo Clinic (United States)
Joseph H. Szurszewski, Mayo Clinic (United States)


Published in SPIE Proceedings Vol. 1808:
Visualization in Biomedical Computing '92

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