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Proceedings Paper

Detection of apoptosis caused by anticancer drug paclitaxel in MCF-7 cells by confocal Raman microscopy
Author(s): H. Salehi; E. Middendorp; A-G. Végh; S-K. Ramakrishnan; C. Gergely; F. J. G. Cuisinier
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Paper Abstract

Confocal Raman Microscopy, a non-invasive, label free imaging technique is used to study apoptosis in living MCF-7 cells. The images are based on Raman spectra of cells components. K-mean clustering was used to determine mitochondria position in cells and cytochrome c distribution inside the cells was based on correlation analysis. Cell apoptosis is defined as cytochrome c diffusion in cytoplasm. Co-localization of cytochrome c is found within mitochondria after three hours of incubation with 10 μM paclitaxel. Our results demonstrate that the presence of paclitaxel at this concentration in the culture media for 3 hours does not induce apoptosis of MCF7 cells via a caspase independent pathway.

Paper Details

Date Published: 19 February 2013
PDF: 10 pages
Proc. SPIE 8594, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications X, 85940F (19 February 2013); doi: 10.1117/12.1000149
Show Author Affiliations
H. Salehi, Lab. Biologie-Santé Nanosciences, Univ. Montpellier 1 (France)
E. Middendorp, Lab. Biologie-Santé Nanosciences, Univ. Montpellier 1 (France)
A-G. Végh, Lab. Biologie-Santé Nanosciences, Univ. Montpellier 1 (France)
The Institute of Biophysics (Hungary)
S-K. Ramakrishnan, Lab. Charles Coulomb, CNRS, Univ. Montpellier 2 (France)
C. Gergely, Lab. Charles Coulomb, CNRS, Univ. Montpellier 2 (France)
F. J. G. Cuisinier, Lab. Biologie-Santé Nanosciences, Univ. Montpellier 1 (France)


Published in SPIE Proceedings Vol. 8594:
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications X
Alexander N. Cartwright; Dan V. Nicolau, Editor(s)

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