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Proceedings Paper

Metabolic changes of cultured DRG neurons induced by adenosine using confocal microscopy imaging
Author(s): Liqin Zheng; Yimei Huang; Jiangxu Chen; Yuhua Wang; Hongqin Yang; Yanding Zhang; Shusen Xie
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Paper Abstract

Adenosine exerts multiple effects on pain transmission in the peripheral nervous system. This study was performed to use confocal microscopy to evaluate whether adenosine could affect dorsal root ganglia (DRG) neurons in vitro and test which adenosine receptor mediates the effect of adenosine on DRG neurons. After adding adenosine with different concentration, we compared the metabolic changes by the real time imaging of calcium and mitochondria membrane potential using confocal microscopy. The results showed that the effect of 500 μM adenosine on the metabolic changes of DRG neurons was more significant than others. Furthermore, four different adenosine receptor antagonists were used to study which receptor mediated the influences of adenosine on the cultured DRG neurons. All adenosine receptor antagonists especially A1 receptor antagonist (DPCPX) had effect on the Ca2+ and mitochondria membrane potential dynamics of DRG neurons. The above studies demonstrated that the effect of adenosine which may be involved in the signal transmission on the sensory neurons was dose-dependent, and all the four adenosine receptors especially the A1R may mediate the transmission.

Paper Details

Date Published: 11 December 2012
PDF: 7 pages
Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 855314 (11 December 2012); doi: 10.1117/12.1000079
Show Author Affiliations
Liqin Zheng, Fujian Normal Univ. (China)
Yimei Huang, Fujian Normal Univ. (China)
Jiangxu Chen, Fujian Normal Univ. (China)
Yuhua Wang, Fujian Normal Univ. (China)
Hongqin Yang, Fujian Normal Univ. (China)
Yanding Zhang, Fujian Normal Univ. (China)
Shusen Xie, Fujian Normal Univ. (China)

Published in SPIE Proceedings Vol. 8553:
Optics in Health Care and Biomedical Optics V
Qingming Luo; Ying Gu; Xingde D. Li, Editor(s)

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