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Proceedings Paper

Measuring intracellular calcium dynamics of HeLa cells exposed to nitric oxide by microplate fluorescence reader
Author(s): Yimei Huang; Jiangxu Chen; Hongqin Yang; Liqin Zheng; Yuhua Wang; Hui Li; Shusen Xie
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Paper Abstract

Nitric oxide (NO) has been reported to have the ability to promote or inhibit the proliferation and metastasis of cancer cells. It appears to have an effect on inducing calcium transient, which participates in essential cellular signaling in the physiological and pathological processes. Our work was intended to study the effects of exogenous NO on intracellular calcium dynamics of HeLa cells with Fluo-3, a calcium fluorescent indicator by microplate fluorescence reader. The results showed that after NO donor was injected into the wells, intracellular Ca2+ fluorescence intensity increased significantly compared with that of control group. Furthermore, the calcium transient activated by NO was mainly due to the calcium release from intracellular calcium stores. These would be helpful to further recognize the role of NO involved in cancer cell proliferation and metastasis.

Paper Details

Date Published: 11 December 2012
PDF: 6 pages
Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 855313 (11 December 2012); doi: 10.1117/12.1000059
Show Author Affiliations
Yimei Huang, Fujian Normal Univ. (China)
Jiangxu Chen, Fujian Normal Univ. (China)
Hongqin Yang, Fujian Normal Univ. (China)
Liqin Zheng, Fujian Normal Univ. (China)
Yuhua Wang, Fujian Normal Univ. (China)
Hui Li, Fujian Normal Univ. (China)
Shusen Xie, Fujian Normal Univ. (China)

Published in SPIE Proceedings Vol. 8553:
Optics in Health Care and Biomedical Optics V
Qingming Luo; Ying Gu; Xingde D. Li, Editor(s)

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