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Optical Engineering

Detector With Charge-Coupled-Device Sensor For Protein Crystallography With Synchrotron X Rays
Author(s): I. Naday; M. G. Strauss; I. S. Sherman; M. R. Kramer; E. M. Westbrook
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Paper Abstract

A two-dimensional detector consisting of a 40-mm-diameter x-ray fluorescing phosphor, coupled with an image intensifier and lens to a CCD image sensor, was developed for protein crystallography with synchrotron x rays. The intense x radiation from a synchrotron source could, with a suitable detector, provide a complete set of diffraction images from a protein crystal before the crystal is damaged by radiation (2 to 3 min). The CCD-based detector was evaluated in an experiment with a rotating anode x-ray generator to determine its suitability in this application. Diffraction patterns from a lysozyme crystal obtained with this detector were compared to those obtained with film. The two images appear to be virtually identical. The flux of 104 x-ray photons/s was observed on the detector at the rotating anode generator. At the 6-GeV synchrotron being designed at Argonne National Laboratory, the flux on an 80 X80 mm2 detector is expected to be >109 x-ray photons/s. The projected design of such a synchrotron detector shows that with a CCD pixel full-well capacity of 7 X105 diffraction peaks containing 3 X 106 x rays could be recorded. With an exposure time of 0.5 s and an additional 0.5 s readout time of a 512 X512 pixel CCD, the data acquisition time per frame would be 1 s so that ninety 1° diffraction images could be obtained, with approximately 1% precision, in less than 3 min.

Paper Details

Date Published: 1 August 1987
PDF: 7 pages
Opt. Eng. 26(8) doi: 10.1117/12.7974150
Published in: Optical Engineering Volume 26, Issue 8
Show Author Affiliations
I. Naday, Argonne National Laboratory (United States)
M. G. Strauss, Argonne National Laboratory (United States)
I. S. Sherman, Argonne National Laboratory (United States)
M. R. Kramer, Argonne National Laboratory (United States)
E. M. Westbrook, Argonne National Laboratory (United States)

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