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Optical Engineering

Ultrafast Energy Transfer Processes In Photosynthetic Systems Probed By Picosecond Fluorescence Spectroscopy
Author(s): Francesco Pellegrino
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Paper Abstract

Picosecond fluorescence spectroscopy was used to investigate the ultrafast energy transfers occurring in the light-harvesting accessory pigment complex of both photosynthetic and nonphotosynthetic leaves of the Norway maple (acer platenoides). The time-resolved fluorescence emission was measured for various wavelength regions both above and below 600 nm at room temperature and 90 K. Excitation was provided by single 6 ps pulses at 530 nm obtained from the frequency-doubled output of a Nd:glass laser, and the fluorescence emission was analyzed with the use of a streak camera optical multichannel analyzer system. Nearly identical decay kinetics were observed in both the nonphotosynthetic and photosynthetic systems. These results highlight the close relationship between the physical configuration of the carotenoid and chlorophyll accessory pigment molecules on the light-harvesting lattice and their energy transfer properties. A trapping model incorporating both photoactive and non-photoactive traps as quenchers of the absorbed excitation energy is proposed. These results question the interpretation of the in vivo fluorescence decay kinetics as being indicative of the photoactive state of the light-harvesting complex.

Paper Details

Date Published: 1 October 1983
PDF: 13 pages
Opt. Eng. 22(5) 225508 doi: 10.1117/12.7973190
Published in: Optical Engineering Volume 22, Issue 5
Show Author Affiliations
Francesco Pellegrino, Sperry Corporation (United States)

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