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Optical Engineering

Virometer: Real Time Virus Detection and Identification in Biological Fluids
Author(s): Tomas Hirschfeld; Myron L. Block
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Paper Abstract

An optical microscopic instrument is described that will allow visual observation and photoelectric detection measurement and classification of moderate concentrations of viruses in biological fluids in a few minutes. The instrument combines fluorescence correlation spectroscopy and ATR fluorescence with special electronics and a correlator-fast Fourier transform data system to measure the size of viral particles while disregarding background objects found in typical biological fluids. Further data processing allows calculation of the nucleic acid mass of individual particles and their total concentration. A novel chemical procedure is employed to differentiate various types of viral nucleic acid. The combination of these measurements is then used to classify a virion without requiring the use of a specific antibody for it. Calibration of the system against test objects and compounds, as well as measurements on several virus types will be shown.

Paper Details

Date Published: 1 August 1977
PDF: 2 pages
Opt. Eng. 16(4) 164406 doi: 10.1117/12.7972061
Published in: Optical Engineering Volume 16, Issue 4
Show Author Affiliations
Tomas Hirschfeld, Block Engineering, Inc. (United States)
Myron L. Block, Block Engineering, Inc. (United States)


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