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Optical Engineering

Degradation of cholesterol crystals in phospholipids
Author(s): Eugen Koren; Mirna Koscec; Robert D. Fugate
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Paper Abstract

Based on previous studies from this laboratory that demonstrated degradation of cholesterol crystals ingested by macrophages in a cell culture system and indicated that intracellular phospholipids could play an important role in mobilization of crystalline cholesterol, the role of each of the three major intracellular phospholipid species in degradation of crystals is further explored. Fluorescently labeled cholesterol crystals are incubated with phospholipids over a period of 5 d. Morphological changes in crystals are monitored using digital imaging fluorescence microscopy, fluorescence redistribution after photobleaching, confocal microscopy, and epifluorescent and phase contrast microscopy. Results clearly demonstrate that all three phospholipids are able to mobilize crystalline cholesterol. However, the mechanisms by which they exert mobilization are different. Sphingomyelin and phosphatidylcholine are found to cause gradual and uniform dissolution of crystals, more or less preserving their original shape. Phosphatidylethanolamine appear to penetrate into the crystal, causing its fragmentation and solubilization. In the mixture of all three phospholipids representing the composition found in macrophages, both of the described mechanisms are working simultaneously.

Paper Details

Date Published: 1 February 1993
PDF: 6 pages
Opt. Eng. 32(2) doi: 10.1117/12.60751
Published in: Optical Engineering Volume 32, Issue 2
Show Author Affiliations
Eugen Koren, Oklahoma Medical Research Foundation (United States)
Mirna Koscec, Oklahoma Medical Research Foundation (United States)
Robert D. Fugate, Univ. of Oklahoma Health Science Ctr. (United States)


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