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Optical Engineering

Intracellular fluorescence polarization, picosecond kinetics, and light-induced reactions of photosensitizing porphyrins
Author(s): Harold K. Seidlitz; Kurt Stettmaier; Jurina M. Wessels; Herbert Schneckenburger
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Paper Abstract

Porphyrin monomers, dimers, and aggregates with various tumor- localizing and photosensitizing properties can be differentiated based on their fluorescence lifetimes. These individual components are shown to possess different degrees of polarization. This fact can be used both for their microscopic imaging and for investigation of the microenvironment of porphyrins within cells and tissues. The spectral bands around 635 and 675 nm were correlated with the emission of porphyrin monomers and aggregates, respectively. The formation of photoprotoporphyrin was not significant during the light-induced porphyrin reaction.

Paper Details

Date Published: 1 July 1992
PDF: 5 pages
Opt. Eng. 31(7) doi: 10.1117/12.57692
Published in: Optical Engineering Volume 31, Issue 7
Show Author Affiliations
Harold K. Seidlitz, GSF-Zentrales Laserlab. (Germany)
Kurt Stettmaier, Gesellschaft fuer Strahlen und Umweltforschung Gmb (Germany)
Jurina M. Wessels, Gesellschaft fuer Strahlen (Germany)
Herbert Schneckenburger, Fachhochschule Aalen (Germany)

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