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Journal of Biomedical Optics

Ratio-based decisions and the quantitative analysis of cDNA microarray images
Author(s): Yidong Chen; Edward R. Dougherty; Michael L. Bittner
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Paper Abstract

Gene expression can be quantitatively analyzed by hybridizing fluor-tagged mRNA to targets on a cDNA microarray. Comparison of gene expression levels arising from cohybridized samples is achieved by taking ratios of average expression levels for individual genes. A novel method of image segmentation is provided to identify cDNA target sites and a hypothesis test and confidence interval is developed to quantify the significance of observed differences in expression ratios. In particular, the probability density of the ratio and the maximum-likelihood estimator for the distribution are derived, and an iterative procedure for signal calibration is developed.

Paper Details

Date Published: 1 October 1997
PDF: 11 pages
J. Biomed. Opt. 2(4) doi: 10.1117/12.281504
Published in: Journal of Biomedical Optics Volume 2, Issue 4
Show Author Affiliations
Yidong Chen, National Institutes of Health (United States)
Edward R. Dougherty, Texas A&M Univ. (United States)
Michael L. Bittner, National Institutes of Health (United States)


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