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Journal of Biomedical Optics

Sensing and reference pads for integrated optical immunosensors
Author(s): Juerg Duebendorfer; Rino E. Kunz; E. Schuermann; Gert L. Duveneck; Markus Ehrat
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Paper Abstract

For integrated optical immunosensors, a much more reliable interpretation of the sensor output signal can be achieved by introducing reference pads near the sensing pads, in order to separate specific from nonspecific effects. The results of theoretical and experimental investigations are reported for immunosensors based on measuring changes of the effective refractive index due to the binding of analyte molecules. The emphasis is on the correction for variations of the light wavelength, the angle of incidence, and the temperature for integrated optical grating coupler chips. Using the binding of rabbit immunoglobulin to immobilized protein A as a regenerable model bioaffinity system, the influence of consecutive assay cycles on the two pads has been investigated. Experiments have been performed using sensor chips consisting of TiO2 waveguiding films on fused silica and on replicated polycarbonate substrates.

Paper Details

Date Published: 1 October 1997
PDF: 10 pages
J. Biomed. Opt. 2(4) doi: 10.1117/12.281487
Published in: Journal of Biomedical Optics Volume 2, Issue 4
Show Author Affiliations
Juerg Duebendorfer, CSEM SA (Switzerland)
Rino E. Kunz, CSEM SA (Switzerland)
E. Schuermann, Paul Scherrer Institute (Switzerland)
Gert L. Duveneck, Novartis Pharma AG (Switzerland)
Markus Ehrat, Novartis Pharma AG (Switzerland)


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