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Journal of Biomedical Optics

Optical tweezers based on near infrared diode laser
Author(s): S. Grego; Ennio Arimondo; Carlo Frediani
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Paper Abstract

Emission from a single-mode 100 mW diode laser at 840 nm is used to create optical tweezers: the trapping laser beam is introduced into a microscope and focused by the objective. The microscope also allows monitoring of the motion of the trapped particles. The optical tweezers were monitored with objectives having different numerical apertures between 0.65 and 1.3. The optical trapping of polystyrene spheres with a radius between 0.11 and 7.45 μm and of biological objects, the flagellated alga Tetraselmis, with typical dimensions of 8×8×13 μm3 were studied. The efficiency of the optical tweezers has been characterized through a parameter Q and compared with theoretical models.

Paper Details

Date Published: 1 July 1997
PDF: 8 pages
J. Biomed. Opt. 2(3) doi: 10.1117/12.275449
Published in: Journal of Biomedical Optics Volume 2, Issue 3
Show Author Affiliations
S. Grego, Niels Bohr Institute (Denmark)
Ennio Arimondo, Univ. di Pisa (Italy)
Carlo Frediani, Istituto Di Biofisica (Italy)


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