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Journal of Biomedical Optics

Cell damage by UVA radiation of a mercury microscopy lamp probed by autofluorescence modifications, cloning assay, and comet assay
Author(s): Karsten Koenig; Tatiana B. Krasieva; Eckhard Bauer; Ursula Fiedler; Michael W. Berns; Bruce J. Tromberg; Karl-Otto Greulich
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Paper Abstract

Cell damage by low-power 365-nm radiation of a 50-W high-pressure mercury microscopy lamp was studied. Exposure of Chinese hamster ovary (CHO) cells to ultraviolet-A (UVA) radiation .10 kJ/m2 resulted in significant modifications of nicotinamide adenine dinucleotide (NADH) attributed autofluorescence and inhibition of cell division. Single-cell gel electrophoresis (comet assay) revealed UVA-induced single-strand DNA breaks. According to these results, UVA excitation radiation in fluorescence microscopy may damage cells. This has to be considered in vital cell microscopy, e.g., in calcium measurements.

Paper Details

Date Published: 2 April 1996
PDF: 6 pages
J. Biomed. Opt. 1(2) doi: 10.1117/12.233373
Published in: Journal of Biomedical Optics Volume 1, Issue 2
Show Author Affiliations
Karsten Koenig, Institute of Molecular Biotechnology (Germany)
Tatiana B. Krasieva, Beckman Laser Institute and Medical Clinic (United States)
Eckhard Bauer, Institute for Molecular Biotechnology (Germany)
Ursula Fiedler, Institut fuer Molekulare Biotechnologie eV (Germany)
Michael W. Berns, Beckman Laser Institute and Medical Clinic (United States)
Bruce J. Tromberg, Beckman Laser Institute and Medical Clinic (United States)
Karl-Otto Greulich, Institut fuer Molekulare Biotechnologie eV (Germany)

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